In situserial Laue diffraction on a microfluidic crystallization device
نویسندگان
چکیده
منابع مشابه
In situ serial Laue diffraction on a microfluidic crystallization device.
Renewed interest in room-temperature diffraction has been prompted by the desire to observe structural dynamics of proteins as they function. Serial crystallography, an experimental strategy that aggregates small pieces of data from a large uniform pool of crystals, has been demonstrated at synchrotrons and X-ray free-electron lasers. This work utilizes a microfluidic crystallization platform f...
متن کاملRoom-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction. Corrigendum
The name of one of the authors in the article by Heymann et al. [(2014), IUCrJ, 1, 349-360] is corrected.[This corrects the article DOI: 10.1107/S2052252514016960.].
متن کاملRoom-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction
An emulsion-based serial crystallographic technology has been developed, in which nanolitre-sized droplets of protein solution are encapsulated in oil and stabilized by surfactant. Once the first crystal in a drop is nucleated, the small volume generates a negative feedback mechanism that lowers the supersaturation. This mechanism is exploited to produce one crystal per drop. Diffraction data a...
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Acetylcholinesterase (AChE) is one of nature's fastest enzymes, despite the fact that its three-dimensional structure reveals its active site to be deeply sequestered within the molecule. This raises questions with respect to traffic of substrate to, and products from, the active site, which may be investigated by time-resolved crystallography. In order to address one aspect of the feasibility ...
متن کاملTime-resolved Laue diffraction of deforming micropillars.
We demonstrate real-time resolved white beam Laue diffraction during compression of micron-sized focused ion beam milled single crystals Au pillars, revealing the dynamical correlation between microstructure and plasticity. The evolution of the Laue patterns of the Au pillars demonstrates the occurrence of crystal rotation and strengthening is explained by plasticity starting on a slip system t...
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ژورنال
عنوان ژورنال: Journal of Applied Crystallography
سال: 2014
ISSN: 1600-5767
DOI: 10.1107/s1600576714023322